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KMID : 0388220070140020136
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Journal of the Korean Rheumatism Association
2007 Volume.14 No. 2 p.136 ~ p.143
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Polymorphisms of TNF-α Gene and TNF Receptor Gene in Behcet’s Disease
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Seo Wook-Jang
Kim Yong-Gil
Moon Hee-Bom
Yoo Bin
Lee Chang-Keun
Park Kyung-Sook
Yang Suk-Kyun
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Abstract
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Methods: DNA of peripheral blood samples was obtained from 43 patients with intestinal Behcet’s disease, 59 patients with Behcet’s disease without gastrointestinal involvement and 60 healthy controls. After polymerase chain reaction (PCR), we examined SNPs of TNF-α gene (??308, ??238) and TNF receptor gene (??1493, ??196, ??1466) by SNaPshot assay. We also analyzed reconstruction of haplotypes of three TNF receptor genes.
Results: No significant difference was observed in the distribution of TNF-α gene (??308, ??238) and TNF receptor gene (??1493, ??196, ??1466) polymorphisms among the groups. Haplotype reconstruction analysis of three TNF receptor genes showed difference in allele group of (T-T-G)(??1493/??196/??1493) between patients with intestinal Behcet’s disease and Behcet’s disease without gastrointestinal involvement (p＜0.05). However, other allele groups revealed no difference among the groups.
Conclusion: The role of TNF-α gene (??308, ??238) polymorphisms and TNF receptor gene (??1493, ??196, ??1466) polymorphisms in the pathogenesis of Behcet’s disease is not supported by this data. Haplotype reconstruction analysis showed that haplotype of T-T-G (TNFR2, ??1493/??196/??1466) in Behcet’s disease may have protective effect on gastrointestinal involvement of this disease.
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KEYWORD
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Behcet’s disease, Single nucleotide polymorphism, Haplotype
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